TABLE 1.
Bacterial strains, plasmids, and bacteriophage
| Strain, plasmid, or bacteriophage | Genotype or relevant characteristicsa | Source or reference |
|---|---|---|
| Strains | ||
| E. coli | ||
| S17-1 | thi pro hsdR recA chr::RP4-2 | 26 |
| MG4λI14 | λ lysogen carrying a chromosomal lasI::lacZ fusion | 24 |
| PK201 | MG1655 (F−) ΔdksA Kanr | EGSC (10)b |
| MC1061 | F−araD139 Δ(ara-leu)7696 galE15 galK16 Δ(lac)X74 rpsL thi | Laboratory collection |
| MC1061D | MC1061 ΔdksA Kanr | This study |
| P. aeruginosa | ||
| PT5 | PAO1 wild-type | Laboratory collection |
| PAO-JP2 | PDO100ΔlasI Tetr Hgr | 19 |
| PAO-RC1 | PT5 ΔdksA Hgr | This study |
| Plasmids | ||
| pBluescript II SK(+) | General-purpose cloning vector, ori (ColE1); Apr | Stratagene |
| pME3088 | Suicide plasmid; Tetr | 32 |
| pVD99.3 | pUCP24 containing dksA on a 960-bp SmaI fragment; Gmr | 2 |
| pRK7813 | RP4-based cosmid vector; Tetr | 9 |
| pRKD1 | dksA in pRK7813; Tetr | This study |
| pRKDE1 | dksA (E. coli) in pRK7813; Tetr | This study |
| pPB101 | dksA in pME3088; Tetr | This study |
| pPB102 | dksA::ΩHg in pME3088; Hgr Tetr | This study |
| pLP170 | Promoterless lacZ fusion vector; Apr | 21 |
| pSW205 | Promoterless lacZ fusion vector; Apr | 7 |
| p101.170 | lasB::lacZ (transcriptional) on pLP170; Apr | This study |
| pECP65 | rhlAB::lacZ (transcriptional) on pLP170; Apr | E. C. Pesci |
| pTS400 | lasB::lacZ (translational) on pSW205; Apr | 18 |
| pECP60 | rhlAB::lacZ (translational) on pSW205; Apr | 20 |
| pPCS1001 | lasR::lacZ (transcriptional) on pLP170; Apr | 20 |
| pPCS223 | lasI::lacZ (transcriptional) on pLP170; Apr | 31 |
| pPCS1002 | rhlR::lacZ (transcriptional) on pLP170; Apr | 20 |
| pLPRI | rhlI::lacZ (transcriptional) on pLP170; Apr | 31 |
| pECP61.5 | rhlAB::lacZ (translational) ptac::rhlR Apr | 19 |
| pECP64 | lasB::lacZ (translational) ptac::lasR Apr | 19 |
| Bacteriophage | ||
| P1 | Transducing phage | S. Raina |
a
Resistance phenotypes: Tet, tetracycline; Ap, ampicillin; Kan, kanamycin; Gm, gentamicin.
b
EGSC, E. coli Genetic Stock Center, Yale University, New Haven, Conn.