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. 2003 Jun;185(12):3583–3595. doi: 10.1128/JB.185.12.3583-3595.2003

FIG. 3.

FIG. 3.

A dnaA(Sx) mutation reduces chromosome content of dnaX+ and Ts strains in glucose-Casamino Acids medium at 39°C. Cells growing exponentially (A, C, and E) or after replication run-out (B, D, and F) were analyzed as described in the Fig. 1 legend. Wild-type (dnaA+ dnaX+) strain AB21 (A and B), dnaA (R432L) dnaX+ strain AB20 (C and D), and dnaA R432L dnaX(Ts) strain EGC23 (E and F) cells were analyzed. In panel F, the peak at less than 1 chromosome/cell was not characterized but might represent cell fragments with trapped DNA.