Phase contrast (A, C, and E) and DAPI-stained (B, D, and F) wild-type strain AB21 (A and B), dnaA (R432L) dnaX+ strain AB20 (C and D), and dnaA R432L dnaX(Ts) strain EGC23 (E and F) cells growing at 39°C. The same microscope fields were examined by both fluorescence and phase microscopy. Bar, 5 μm.