TABLE 4.
A dnaA suppressor mutation reduces the origin-to-terminusa ratio, as measured by real-time PCR analysis
| Medium and strain | dnaA | dnaX | Temp (°C) | Marker frequency
|
|
|---|---|---|---|---|---|
| oriC/xasA (SD)b | proA/xasA (SD) | ||||
| Glucose-Casamino Acids | |||||
| AB21 | + | + | 34 | 3.00 (0.40) | 2.04 (0.14) |
| 39 | 3.47 (0.49) | ||||
| AB20 | Sx | + | 34 | 1.77 (0.10) | 1.36 (0.12) |
| 39 | 1.83 (0.22) | ||||
| AB27 | + | Ts | 30 | 3.49 (0.32) | 2.09 (0.34) |
| EGC23 | Sx | Ts | 34 | 1.84 (0.16) | 1.32 (0.05) |
| 39 | 1.50 (0.10) | ||||
| Glycerol | |||||
| AB21 | + | + | 34 | 2.00 (0.19) | 1.45 (0.07) |
| AB20 | Sx | + | 34 | 1.48 (0.07) | 1.22 (0.05) |
| AB27 | + | Ts | 30 | 1.79 (0.13) | 1.43 (0.10) |
| EGC23 | Sx | Ts | 34 | 1.16 (0.10) | 1.16 (0.10) |
a
The xasA marker was used as an indicator of the terminus.
b
Standard deviations for six to nine determinations.