FIG. 2.

Expression of Asc10 insertion mutants in TX5128. (A) The insertions that resulted in aggregation defects when expressed in OG1RF are shown on a linear map of the gene encoding Asc10. The boxed insertions are those that maintained an aggregation defect when expressed in TX5128. (B) A Western blot of lysozyme surface extracts of insertions in the putative Asc10 aggregation domain expressed in TX5128 confirms full-length protein expression. (7517, wild-type Asc10). Molecular mass markers are indicated to the left of the blot.