FIG. 5.

GelE-expressing strains are more autolytic. (A) Autolysis of the single protease mutants compared to that of OG1RF. TX5264 was complemented with GelE expressed from plasmid 3614 (plasmid 3535 is the vector control). (B) Autolysis of the double protease mutant TX5128 compared with that of OG1RF. TX5128 was also complemented with plasmid 3614. The data are plotted at each point as the percentage of the initial OD₆₃₀. Each point represents the mean for four to six independent cultures, and the error bars depict the standard errors of the means.