TABLE 2.

Squid colonization by flrA mutant and complementing strains

Strain	No. of animals	% Colo- nizeda	Symbiotic luminescenceb				CFU/light organc (104)
			12 h	24 h	36 h	48 h
ES114 pVO8	15	100	100	100	100	100	14 ± 2
DM126 pVO8	15	0	<0.1	<0.1	<0.1	<0.1	<0.001
DM159 pVO8	10	0	<0.1	<0.1	<0.1	<0.1	NDd
ES114 pDM58	30	94	100	100	100	100	9 ± 1
DM126 pDM58	30	46	<0.1	34	59	27	0.16 ± 0.5
DM159 pDM58	20	35	ND	27	ND	27	0.9 ± 0.2

^aColonized animals were defined as those displaying luminescence at 24 h.

^bRelative to the levels of the cognate ES114 strain (set at 100).

^cColonization levels determined at 48 h postinoculation.

^dND, not determined.