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. 2006 Jul 14;91(7):2626–2635. doi: 10.1529/biophysj.106.088369

FIGURE 1.

FIGURE 1

Concept behind confocal SPCE microscope. The fluorophores are placed on a metal-coated coverslip and excited with green light at an SPR angle. The excitation energy couples to the surface plasmons and radiates to the glass prism (red) as a surface of a cone with half-angle equal to the SPCE angle. Metal can be a thin layer of Al (20 nm thick) or Ag or Au (50 nm thick). The picture of the directional emission is taken from a real experiment.