TABLE 1.
Qualitative comparison of myofibrillar images with different coverslips
Excitation | Glass uncoated | Quartz uncoated | Sapphire uncoated | Olympus uncoated | Sapphire coated with gold | Olympus coated with gold | Olympus coated with silver |
---|---|---|---|---|---|---|---|
TIRF | +++* | +++ | +++ | ++ | ++(dim) | ++ (dim) | ++ (dim) |
EPI | ++† | ++† | ++† | ++† | ++ | + | − |
SPCE | NP‡ | NP | NP | NP | +++ | +++ | ++ |
Excitation was with Argon laser at 488 nm.
+++, very good; ++, good; +, fair; −, inferior.
Images are good because myofibril is thin (∼0.5 μm).
Not possible.