Skip to main content
. 2006 Jul 14;91(7):2626–2635. doi: 10.1529/biophysj.106.088369

TABLE 1.

Qualitative comparison of myofibrillar images with different coverslips

Excitation Glass uncoated Quartz uncoated Sapphire uncoated Olympus uncoated Sapphire coated with gold Olympus coated with gold Olympus coated with silver
TIRF +++* +++ +++ ++ ++(dim) ++ (dim) ++ (dim)
EPI ++ ++ ++ ++ ++ +
SPCE NP NP NP NP +++ +++ ++

Excitation was with Argon laser at 488 nm.

*

+++, very good; ++, good; +, fair; −, inferior.

Images are good because myofibril is thin (∼0.5 μm).

Not possible.