Skip to main content
. 2006 Jun 30;91(7):2687–2698. doi: 10.1529/biophysj.106.086181

FIGURE 5.

FIGURE 5

A binary mixture of Rhodamine 6G and Rhodamine 110 resolved by dual-color TIFCA. A 544-nm dichroic mirror was used to separate the emission into two detection channels. The data was taken with a sampling time of 20 μs for 130 s. Fitting the cumulants up to fourth-order to a single-species model (data not shown) returns a reduced χ2 value of 478. A two-species model fits the experimental cumulants within error (χ2 of 0.93). The parameters recovered from the two-species fit are: λA1 = 34,000 cpsm, λB1 = 9300 cpsm, τd1 = 48 μs, and N1 = 1.07 for Rhodamine 6G and λA2 = 11,000 cpsm, λB2 = 24,000 cpsm, τd2 = 49 μs, and N2 = 1.58 for Rhodamine 110.