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. 2006 Jun 23;91(7):2532–2542. doi: 10.1529/biophysj.106.085688

FIGURE 3.

FIGURE 3

(A) DPPC lipid sample imaged at 50°C showing lipid bilayer with gel and fluid phase separated domains. Lighter areas correspond to higher regions of gel phase, whereas darker areas correspond to lower regions of fluid phase. Scale bar, 0.2 μm. (B) Image of the same region in panel A obtained at 60°C. No phase-separated domains are observed due to the complete transition of the bilayer to the fluid phase. Scale bar, 0.2 μm (C) Histogram of height difference between the gel and fluid phase regions in panel A using the masking method described above in Fig. 2 F.