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Time course analysis of denitrase activity. (A) Each piece of membrane containing nitrated histone H1.2 was incubated with cell lysate from LPS-treated RAW 264.7 cells for various lengths of time. The signals for the 1D membrane assay are shown. (B) The signals were quantified densitometrically and plotted according to the incubation time. Note that the y axis is logarithmic. A decrease of signal in accordance with the incubation time was seen.
