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Inducible expression of AHR target genes. Northern blot analysis of AHR-regulated CYP1A1 and CYP1A2 was performed. Six-week-old homozygous hAHR, Ahrd/d, and Ahrb-1/b-1 mice were treated with 80 mg/kg 3-MC (A) or 100 μg/kg TCDD (B). Total hepatic RNA was isolated 24 h after treatment and subjected to Northern analysis (10 μg/lane). Equal loading was confirmed by the abundance of GAPDH transcripts.
