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. 2003 Apr 29;100(10):5670–5675. doi: 10.1073/pnas.1031504100

Figure 2.

Figure 2

Comparison of first-derivative EPR absorption spectra of VO2+. (Left) a, dUTPase–VO2+–dUDP complex; b, identical conditions to those in a but after addition at 5°C of a 10-fold excess of Mg2+; and c, dUTPase–(VO2+-chelate)–dUDP complex. (Right) An expanded comparison of the −3/2⊥ feature of the EPR spectrum (indicated by an arrow in Left) of the VO(acac)2–dUDP mixture in the absence (black spectrum) and presence (red spectrum) of dUTPase. The intensity ratios of 1:2:1, the three hyperfine lines with ap = 7.19 G, and the total line width of 19.78 G are also indicated in red. Molar ratios of enzyme–VO2+–dUDP or of enzyme–VO(acac)2–dUDP were 1.25:1:1, respectively, at a dUTPase concentration of 1.35 × 10−3 M buffered to pH 7 in 0.1 M NaCl with 0.01 M Hepes.