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Comparison of the influence of divalent metal ions on steady-state kinetic parameters governing hydrolysis of dUTP catalyzed by recombinant E. coli dUTPase
| Metal ion | kcat (s−1) | kcat/Km (M−1⋅s−1 × 106) |
|---|---|---|
| No metal ion | 4.5 ± 0.1 | 0.49 ± 0.1 |
| Mg2+ | 14.7 ± 2.2 | 25.1 ± 7.4 |
| Mn2+ | 14.5 ± 2.2 | — |
| VO2+ | 27.3 ± 4.1 | 2.8 ± 0.9 |
| VO(acac)2 | 24.2 ± 3.6 | 2.4 ± 0.7 |
—, Not determined.
