Table 1.
Kinetic constants for the cleavage of Neu5Ac
| kcat (per subunit), s−1 | KNeu5Ac, mM | kcat/KNeu5Ac, s−1⋅mM−1 | |
|---|---|---|---|
| NAL | 7.7 ± 0.5 | 2.5 ± 0.2 | 3.1 |
| L142R | 12.1 ± 0.9 | 13.6 ± 1.3 | 0.9 |
| L142R/Y190D/E192A | 6.8 ± 1.6 | 125 ± 36 | 0.05 |
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