Figure 1.

Genomic context of the rpmH–rnpA genes in T. thermophilus HB8. The rpmH (L34) reading frame is shaded, and the amino acid sequence for the overlapping rnpA reading frame is given and numbered beneath the nucleotide sequence. Start and stop codons are outlined by round and square boxes, respectively. Promoter −35 and −10 elements identified by Maseda and Hoshino (ref. 15; National Center for Biotechnology Information nucleotide gi|971299|) or putative (dotted lines) are marked below the sequence. livG, ORF1,2: adjacent genes. The sequence encoding the potential RNA hairpin 3′ of rpmH is marked by the bulged line. The recombinant versions of C5₁, C5₅₁, and C5₈₇ (starting points indicated by arrows) tested for RNase P activity additionally carried an N-terminal His tag.