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. 2003 Apr 28;100(10):5724–5729. doi: 10.1073/pnas.0931462100

Figure 2.

Figure 2

Endonucleolytic processing of 5′ 32P-labeled ptRNAGly by RNase P holoenzymes at 37°C. (A) RNase P activity test in the presence of protein A Sepharose beads coated with antibodies from three different sera (preimmune serum and antisera specific for T. thermophilus transcription factor NusG or C587) and preincubated with a T. thermophilus DEAE chromatography fraction enriched in RNase P activity. (B) Activation of T. thermophilus RNase P RNA (400 nM) by C551 (80 nM); assay conditions were 37°C, 50 mM Mes, 0.1 M NH4OAc, 10 mM Mg(OAc)2, pH 6.0, < 1 nM ptRNAGly.