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. 2003 Apr 30;100(10):5754–5759. doi: 10.1073/pnas.2225470100

Figure 1.

Figure 1

Oxidative folding of AAI at pH 8.5, 25°C/100 mg of AAI per liter–1 of refolding buffer (100 mM NH4OAc/1M GdnHCl/1 mM cysteine/0.05 mM cystine/2 mM EDTA). (A) Time-resolved RP-HPLC chromatograms. (Inset) The fold and disulfide connectivity of native AAI. (B) Species abundances are calculated from the peak intensities of the detector readings at 214 nm. (Insets) The disulfide connectivity of the native state and main folding intermediate (MFI), the latter being deduced from the data in Table 1.