Figure 5.

Transplantation of SHED into the brain. (A) Diagram indicating injection of SHED into the dentate gyrus of the hippocampus. (B) SHED were cultured in the neural-differentiation medium as described in Materials and Methods for 1 week, after which 5,000 cells in 0.5 μl of PBS were injected into the dentate gyrus of the hippocampus of immunocompromised mice. After 10 days, the brain was fixed and prepared for immunofluorescence staining with NFM and human-specific anti-mitochondrial antibody. The anti-mitochondrial antibody immunostaining showed human SHED (arrows, green) in the dentate gyrus of the hippocampus with coexpression of NFM (arrows, red). In merged images, coexpression of human mitochondria and NFM showed colocalization of antigen expression as indicated by yellow fluorescence (arrows). (Magnification, ×20.)