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Adipogenic differentiation of SHED. (A) Cultured SHED formed Oil red O-positive lipid clusters after 5 weeks of induction in the presence of 0.5 mM isobutylmethylxanthine, 0.5 μM hydrocortisone, and 60 μM indomethacin. (B) A significant up-regulation of peroxisome proliferator-activated receptor-γ2 (PPARγ2) and lipoprotein lipase (LPL) was observed in the group induced with the adipogenic mixture (Adip) as compared with the control group (Cont) by RT-PCR.
