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. 2003 Apr 28;100(10):5834–5839. doi: 10.1073/pnas.0935626100

Figure 1.

Figure 1

Wnt3A and Wnt8 are early responses in differentiating P19CL6 cells. (A) Gene expression was determined by QRT-PCR, normalized to GAPDH, and represented as relative copy number. (B) Western blot, showing induction of GATA4 and sarcomeric MHCs vs. tubulin as the control. (C) Immunocytochemistry, showing induction of sarcomeric MHCs. Cells were cultivated for 12 days with (Right) or without (Left) 1% DMSO, and stained with MF20 (red) and 4′,6-diamidino-2-phenylindole (blue). (Bar = 50 μm.) (D) FGF8 expression by RT-PCR.