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. 2003 Apr 28;100(10):5902–5907. doi: 10.1073/pnas.0730858100

Figure 3.

Figure 3

Localization of GFP-tagged reference OCT1, OCT1-G465R, and OCT1-R488M in Madin–Darby canine kidney (MDCK) cells. MDCK cells were transfected with plasmids eGFP-OCT1, eGFP-OCT1-G465R, eGFP-OCT1-R488M, and eGFP, respectively. Stable clones were selected with G418. Cells were polarized by growth on filters for 7 days. The cells were fixed, permeabilized, stained for actin with Texas-red-conjugated X phalloidin, and visualized by confocal fluorescence microscopy. A horizontal section (Upper) and a vertical section with the apical membrane on top (Lower) are shown for each set of transfected cells. (Upper Left) eGFP-OCT1. (Upper Right) eGFP-OCT1-G465R. (Lower Left) eGFP-OCT1-R488M. (Lower Right) eGFP.