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Identification of tumor-specific Parkin transcripts. (a) PCR amplification of tumor and normal ovarian cDNA. Samples UPN 66 and UPN 223 possess both wild-type and aberrant transcripts, whereas UPN 323 and normal ovaries 1 and 2 possess only wild-type Parkin. (b) Sequence analysis of aberrant Parkin transcripts. A schematic representation of each variant is shown, along with the resulting truncated protein isoform. Also indicated is the location of primer pairs used for both analyses.
