Figure 2.

Schematic diagram of the multigene assembly process. Only two assembly cycles are shown: the first to deliver the MAR and the second to add the GNA gene into the acceptor vector pYLTAC747H. The linkers LS and LV were prepared with phosphorylated oligonucleotides 5′-gcggccgcttat-3′ and 5′-gcggccgcgcac-3′, respectively, whose self-pairing cores form a NotI site with compatible ends extending outward for their intended ligation partners.