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. 2003 Apr 29;100(10):5986–5990. doi: 10.1073/pnas.1035999100

Figure 4.

Figure 4

Enhanced OX40 and CD40L expression on T cells in IL-1Ra−/− mice. CD4+ and OX40+ cell infiltration was observed in synovial tissues of IL-1Ra−/− mice (A–C) but not in wild-type mice (D). Both OX40 and CD40L expression on CD4+ T cells was enhanced in IL-1Ra−/− mice compared with wild-type mice after immunization with IIC (E). (A) Synovial tissues of IL-1Ra−/− mice; hematoxylin/eosin (H&E) staining (magnification, ×25). (B) Synovial tissues of IL-1Ra−/− mice stained with anti-CD4 mAb (magnification, ×100). Arrow shows positive cells. (C) Synovial tissues of IL-1Ra−/− mice stained with anti-OX40 mAb (magnification, ×100). Arrows show positive cells. (D) Synovial tissues of wild-type mice; H&E staining (magnification, ×25). (E) Seven days after immunization with IIC, LN cells were cultured in the absence or presence of 20 μg/ml IIC for 24 (CD40L expression) or 72 h (OX40 expression). Then, CD40L and OX40 expression in CD4+ population was analyzed by flow cytometry. Shaded area shows an isotype-matched control Ig staining.