Table 1.

Sequence of GP I of LCMV-WE wild-type, first- and second-generation nAb-escape mutants

Virus was isolated from blood of infected DBA/2 mice and plaque-purified twice. First-generation nAb-escape mutant viruses were isolated from six mice infected with LCMV-WE (2 × 10⁶ pfu) 90 days after infection and named V1–V6. Second-generation nAb-escape mutant viruses were isolated from seven mice 110 days after infection with 2 × 10⁴ pfu of first-generation nAb mutants (V1–V6) and named V1.1–V1.3, V2.1–V2.3, etc. Several subclones derived from one mouse are shown. Subclones V3.1–V3.6 and subclones V3.7–V3.9 are from two different mice infected with mutant V3. Similarly subclones V4.1–4.3 and subclones V4.4–V4.6 are from two different mice infected with mutant V4. LCMV-WE are bold, first-generation nAb-escape mutants are underlined, and second-generation nAb-escape mutants are in italics. 

^*Known potential glycosylation sites.