Skip to main content
. 2003 Apr 29;100(10):6045–6050. doi: 10.1073/pnas.1030790100

Figure 3.

Figure 3

SPARC-null and wild-type mice do not exhibit significant differences in overall body weight but do show increased levels of serum leptin with advancing age. (A) SPARC-null (●) and wild-type (□) mice housed under identical conditions and fed a 4% fat chow diet ad libitum did not show differences in overall weight gain over time. Ten mice or more were used to generate each time point. Error bars represent the standard deviation of the mean. (B) SPARC-null mice show increased levels of serum leptin with advancing age. Wild-type (open bars) and SPARC-null (black bars) mice were subjected to cardiac puncture 4–6 h after food deprivation. Whereas insulin, glucose, cholesterol, high-density lipoprotein, and triglyceride levels showed no significant differences between genotypes, leptin levels at older ages were increased substantially over those of age-matched, wild-type controls. Leptin levels (ng/ml) were normalized to individual body weights and are expressed as percentages of total body weight. The average weights of the mice at 10 weeks were 22.4 g (±1) (wild type) and 22.8 g (±1.4) (SPARC-null) and at >6 months 22.9 g (±3) (wild type) and 24.7 g (±3.4) (SPARC-null). Four mice for each genotype at 10 weeks and at least seven mice at >6 months contributed to the results. Error bars represent the standard error of the mean. *, P = 0.003 for >6 months.