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. 2003 Apr 29;100(10):6045–6050. doi: 10.1073/pnas.1030790100

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Copyright © 2003, The National Academy of Sciences

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SPARC-null epididymal fat pads contain significantly more large adipocytes than wild-type fat pads. Fat pads were treated with collagenase, and adipocytes were fractionated by centrifugation. Isolated adipocytes were photographed on a hemocytometer slide ( , wild-type adipocytes; ■, SPARC-null adipocytes), and captured images were imported into nih IMAGE software for quantification. (Scale bars, 200 μm.) Wild-type (423 cells, gray bars) and SPARC-null (717 cells, black bars) adipocytes contributed to the distribution. The histogram was generated from the data reported in Tables 2 and 3.

Inline graphic — SPARC-null epididymal fat pads contain significantly more large adipocytes than wild-type fat pads. Fat pads were treated with collagenase, and adipocytes were fractionated by centrifugation. Isolated adipocytes were photographed on a hemocytometer slide ( , wild-type adipocytes; ■, SPARC-null adipocytes), and captured images were imported into nih IMAGE software for quantification. (Scale bars, 200 μm.) Wild-type (423 cells, gray bars) and SPARC-null (717 cells, black bars) adipocytes contributed to the distribution. The histogram was generated from the data reported in Tables 2 and 3.