Figure 2.

(A) cDNA prepared from the index primary renal neoplasm was subjected to 5′ RACE with three antisense primers. Agarose gel electrophoresis reveals RACE products. Predicted PCR products derived from native TFEB message migrate with a smaller molecular weight than the novel PCR products (bands outlined with dotted line). (B) Primary renal tumor genomic DNA as well as cloned RACE product were subjected to PCR with an upstream primer derived from the novel non-TFEB sequence and a downstream primer derived from TFEB. Both DNA samples yielded identically sized PCR products.