FIG. 1.
Analysis of ileS2-carrying plasmid types by restriction endonuclease analysis and Southern hybridization. (A) HindIII restriction patterns of plasmid types extracted from S. aureus transconjugants of Hi-Mupr MRSA isolates. ▸, ileS2-hybridizing fragments. (A1) Southern hybridization with the ileS2-containing DNA as a probe, showing the different ileS2 polymorphs (A to E). (A2) Southern hybridization using the traK DNA fragment as probe. Lanes 1 through 9, plasmid types pMUP1 to pMUP9. λ, lambda phage DNA digested with HindIII and digoxigenin labeled as a molecular size standard. Numbers on the right correspond to the molecular sizes (in base pairs) of λ DNA HindIII restriction fragments.