TABLE 3.
Structural group, ileS2 locus polymorph, and clonal complex distribution of Hi-Mupr plasmid types
| Structural group | ileS2 locus polymorph | Size(s) (kb) of restriction fragments hybridizing with the ileS2 probea | ileS2-carrying plasmid type (kb)b | No. of isolates (%) | MRSA clone(s) | Clonal complex(es) |
|---|---|---|---|---|---|---|
| S1 | A | 4.3 | pMUP4 (26) | 4 (8.3) | ST146-IVA, ST36-II | CC5, CC30 |
| pMUP8 (41) | 2 (4.2) | ST36-II | CC30 | |||
| S2 | B | 5.0 | pMUP3 (34) | 2 (4.2) | ST36-II | CC30 |
| C | 4.7, 5.0 | pMUP7 (39) | 1 (2.1) | ST36-II | CC30 | |
| S3 | D | 5.2 | pMUP1 (33) | 11 (22.9) | ST247-IA, ST36-II | CC8, CC30 |
| pMUP9 (35) | 1 (2.1) | ST36-II | CC30 | |||
| S4 | E | 5.5 | pMUP2 (40) | 21 (43.8) | ST125-IVA, ST247-IA, ST22-IV | CC5, CC8, CC22 |
| pMUP5 (47) | 3 (6.2) | ST36-II | CC30 | |||
| pMUP6 (46) | 3 (6.2) | ST36-II | CC30 |
a
Aproximated sizes by comparison with a lambda HindIII marker.
b
Plasmid sizes were determined by summation of restriction fragments using agarose gel electrophoresis following separate digestions with EcoRI and HindIII.