TABLE 1.
Descriptions of oligonucleotide primers
| Primer | Sequence (5′-3′) | Positions (gene)a | Purpose |
|---|---|---|---|
| AADA1F | ATGAGGGAAGCGGTGATCGC | 1 to 20 (pVE46 aadA1) | Amplification of aadA1, aadA1 RT-PCR |
| AADA1R | CGGCTTGAACGAATTGTTAG | 814 to 795 (pVE46 aadA1) | Amplification of aadA1 |
| AADA1RTR | TTTGCCGACTACCTTGGTGA | 789 to 770 (pVE46 aadA1) | aadA1 RT-PCR |
| AADAPROF | GACATAAAACGCTCCTTGTC | −1486 to −1467 (pVE46 aadA1) | Amplification of aadA1 promoter |
| AADAPROR | CACAATTCTTCCTCAGAGGT | −1148 to −1167 (pVE46 aadA1) | Amplification of aadA1 promoter |
| APHAPROF | GAGTGAGGCGATTGGAAACC | −236 to −217 (RP1 aphA) | Amplification of aphA 5′ end and aphA promoter |
| APHAPROR | GAGCATTCGCTCACTGGGAT | 419 to 400 (RP1 aphA) | Amplification of aphA 5′ end and aphA promoter |
| APHAF | AACGGCATATCAAGTGCTGA | 312 to 331 (RP1 aphA) | Amplification of aphA 3′ end, aphA RT-PCR |
| APHAR | GAAAAGTTCGTCCAGCAGGA | 813 to 794 (RP1 aphA) | Amplification of aphA 3′ end, aphA RT-PCR |
| ORITF | CCCTGATACTTTTGGGCTTC | 1527 to 1546 (pVE46 oriT) | Amplification of oriT |
| ORITR | ACCGATTACCTCCTGAAACC | 114 to 94 (pVE46 oriT) | Amplification of oriT |
| OXA2F | TTGGGCATTAAGGAAAAGTT | −21 to −2 (pVE46 blaOXA-2) | Amplification of blaOXA-2 |
| OXA2R | GTTGAAGTAACCGGCGCTGC | 875 to 859 (pVE46 blaOXA-2) | Amplification of blaOXA-2 |
| OXA2PROF | ATGCCTCGACTTCGCTGCTG | −347 to −328 (pVE46 blaOXA-2) | Amplification of blaOXA-2 promoter |
| OXA2PROR | TGGATGCCCGAGGCATAGAC | −111 to −130 (pVE46 blaOXA-2) | Amplification of blaOXA-2 promoter |
| OXA2RTF | TTCAAGCCAAAGGCACGATAG | 113 to 133 (pVE46 blaOXA-2) | blaOXA-2 RT-PCR |
| OXA2RTR | TCCGAGTTGACTGCCGGGTTG | 815 to 795 (pVE46 blaOXA-2) | blaOXA-2 RT-PCR |
| RPSLF | CTCGCAAAGTTGCGAAAAGC | 38 to 57 (E. coli rpsL) | rpsL RT-PCR |
| RPSLR | TTCACGCCATACTTGGAACG | 359 to 340 (E. coli rpsL) | rpsL RT-PCR |
| SUL1F | CTTTGTAGGTATGGGGCTCA | −81 to −62 (pVE46 sul1) | Amplification of sul1 |
| SUL1R | TGACGAGCCCAGCATGTCTG | 916 to 897 (pVE46 sul1) | Amplification of sul1 |
| SUL1PROF | CGCTGGGTTTGCCGTTTCTC | −694 to −675 (pVE46 sul1) | Amplification of sul1 promoter |
| SUL1PROR | TCTAGCCGCCGGCTCTCATC | 67 to 48 (pVE46 sul1) | Amplification of sul1 promoter |
| SUL1RTF | CCGATATTGCTGAGGCGGACT | 338 to 358 (pVE46 sul1) | sul1 RT-PCR |
| SUL1RTR | CCAACGCCGCTTCAGCTT | 604 to 586 (pVE46 sul1) | sul1 RT-PCR |
| TEMF | ATGAGTATTCAACATTTCCG | 1 to 20 (RP1 blaTEM-2) | Amplification of blaTEM-2, blaTEM-2 RT-PCR |
| TEMR | GACGCTCAGTGGAACGAAAA | 1000 to 981 (RP1 blaTEM-2) | Amplification of blaTEM-2 |
| TEMPROF | TTAGACGTCAGGTGGCACTT | −139 to −120 (RP1 blaTEM-2) | Amplification of blaTEM-2 promoter |
| TEMPROR | GAGCAAAAACAGGAAGGCAA | 79 to 60 (RP1 blaTEM-2) | Amplification of blaTEM-2 promoter |
| TEMRTR | CCAATGCTTAATCAGTGACG | 858 to 839 (RP1 blaTEM-2) | blaTEM-2 RT-PCR |
| TETA1F | AGTAGAGCGCTGGCTGTTGC | −218 to −199 (pVE46 tetA) | Amplification of tetA 5′ end and tetA promoter |
| TETA1R | CATACCCACGCCGAAACAAG | 432 to 413 (pVE46 tetA) | Amplification of tetA 5′ end and tetA promoter |
| TETA2F | GTTGCTGGCGCCTATATCGC | 343 to 362 (pVE46 tetA) | Amplification of tetA 3′ end |
| TETA2R | TCAGGTCGAGGTGGCCCGAC | 1176 to 1157 (pVE46 tetA) | Amplification of tetA 3′ end |
| TETRF | TTCAGCTAGGTGACTTTGCT | −64 to −45 (pVE46 tetR) | Amplification of tetR |
| TETRR | GTGCCTGACTGCGTTAGCAA | 746 to 727 (pVE46 tetR) | Amplification of tetR |
| TETARTF | ACAATGCGCTCATCGTCATC | 11 to 30 (pVE46 tetA) | tetA RT-PCR |
| TETARTR | GGCGCCTACAATCCATGC | 1125 to 1108 (pVE46 tetA) | tetA RT-PCR |
| RP1TET1AF | CGGCTGCAACTTTGTCATGC | −123 to −104 (RP1 tetA) | Amplification of tetA 5′ end |
| RP1TETA1R | CGCCGAAAATGACCCAAAGC | 712 to 693 (RP1 tetA) | Amplification of tetA 5′ end |
| RP1TETA2F | CCGCTCAGCTTCGTTCGGTG | 595 to 614 (RP1 tetA) | Amplification of tetA 3′ end |
| RP1TETA2R | CGATCAGCGATCGGCTCGTT | 1202 to 1183 (RP1 tetA) | Amplification of tetA 3′ end |
| RP1TETRTETPROF | GCAGCGGTCCTGATCAATCG | 663 to 644 (RP1 tetR) | Amplification of tetA promoter and tetR |
| RP1TETRTETPROR | CTCCGCTGGTCCGATTGAAC | −20 to −39 (RP1 tetA) | Amplification of tetA promoter and tetR |
| RP1TETARTF | CGGCCAATCTTGCTCGTCTC | 217 to 236 (RP1 tetA) | tetA RT-PCR |
| RP1TETARTR | CCTGCCTGGACAACATTGCT | 982 to 936 (RP1 tetA) | tetA RT-PCR |
a
Positions are relative to the start codon of the gene.