TABLE 1.
Primer | Sequence (5′-3′) | Positions (gene)a | Purpose |
---|---|---|---|
AADA1F | ATGAGGGAAGCGGTGATCGC | 1 to 20 (pVE46 aadA1) | Amplification of aadA1, aadA1 RT-PCR |
AADA1R | CGGCTTGAACGAATTGTTAG | 814 to 795 (pVE46 aadA1) | Amplification of aadA1 |
AADA1RTR | TTTGCCGACTACCTTGGTGA | 789 to 770 (pVE46 aadA1) | aadA1 RT-PCR |
AADAPROF | GACATAAAACGCTCCTTGTC | −1486 to −1467 (pVE46 aadA1) | Amplification of aadA1 promoter |
AADAPROR | CACAATTCTTCCTCAGAGGT | −1148 to −1167 (pVE46 aadA1) | Amplification of aadA1 promoter |
APHAPROF | GAGTGAGGCGATTGGAAACC | −236 to −217 (RP1 aphA) | Amplification of aphA 5′ end and aphA promoter |
APHAPROR | GAGCATTCGCTCACTGGGAT | 419 to 400 (RP1 aphA) | Amplification of aphA 5′ end and aphA promoter |
APHAF | AACGGCATATCAAGTGCTGA | 312 to 331 (RP1 aphA) | Amplification of aphA 3′ end, aphA RT-PCR |
APHAR | GAAAAGTTCGTCCAGCAGGA | 813 to 794 (RP1 aphA) | Amplification of aphA 3′ end, aphA RT-PCR |
ORITF | CCCTGATACTTTTGGGCTTC | 1527 to 1546 (pVE46 oriT) | Amplification of oriT |
ORITR | ACCGATTACCTCCTGAAACC | 114 to 94 (pVE46 oriT) | Amplification of oriT |
OXA2F | TTGGGCATTAAGGAAAAGTT | −21 to −2 (pVE46 blaOXA-2) | Amplification of blaOXA-2 |
OXA2R | GTTGAAGTAACCGGCGCTGC | 875 to 859 (pVE46 blaOXA-2) | Amplification of blaOXA-2 |
OXA2PROF | ATGCCTCGACTTCGCTGCTG | −347 to −328 (pVE46 blaOXA-2) | Amplification of blaOXA-2 promoter |
OXA2PROR | TGGATGCCCGAGGCATAGAC | −111 to −130 (pVE46 blaOXA-2) | Amplification of blaOXA-2 promoter |
OXA2RTF | TTCAAGCCAAAGGCACGATAG | 113 to 133 (pVE46 blaOXA-2) | blaOXA-2 RT-PCR |
OXA2RTR | TCCGAGTTGACTGCCGGGTTG | 815 to 795 (pVE46 blaOXA-2) | blaOXA-2 RT-PCR |
RPSLF | CTCGCAAAGTTGCGAAAAGC | 38 to 57 (E. coli rpsL) | rpsL RT-PCR |
RPSLR | TTCACGCCATACTTGGAACG | 359 to 340 (E. coli rpsL) | rpsL RT-PCR |
SUL1F | CTTTGTAGGTATGGGGCTCA | −81 to −62 (pVE46 sul1) | Amplification of sul1 |
SUL1R | TGACGAGCCCAGCATGTCTG | 916 to 897 (pVE46 sul1) | Amplification of sul1 |
SUL1PROF | CGCTGGGTTTGCCGTTTCTC | −694 to −675 (pVE46 sul1) | Amplification of sul1 promoter |
SUL1PROR | TCTAGCCGCCGGCTCTCATC | 67 to 48 (pVE46 sul1) | Amplification of sul1 promoter |
SUL1RTF | CCGATATTGCTGAGGCGGACT | 338 to 358 (pVE46 sul1) | sul1 RT-PCR |
SUL1RTR | CCAACGCCGCTTCAGCTT | 604 to 586 (pVE46 sul1) | sul1 RT-PCR |
TEMF | ATGAGTATTCAACATTTCCG | 1 to 20 (RP1 blaTEM-2) | Amplification of blaTEM-2, blaTEM-2 RT-PCR |
TEMR | GACGCTCAGTGGAACGAAAA | 1000 to 981 (RP1 blaTEM-2) | Amplification of blaTEM-2 |
TEMPROF | TTAGACGTCAGGTGGCACTT | −139 to −120 (RP1 blaTEM-2) | Amplification of blaTEM-2 promoter |
TEMPROR | GAGCAAAAACAGGAAGGCAA | 79 to 60 (RP1 blaTEM-2) | Amplification of blaTEM-2 promoter |
TEMRTR | CCAATGCTTAATCAGTGACG | 858 to 839 (RP1 blaTEM-2) | blaTEM-2 RT-PCR |
TETA1F | AGTAGAGCGCTGGCTGTTGC | −218 to −199 (pVE46 tetA) | Amplification of tetA 5′ end and tetA promoter |
TETA1R | CATACCCACGCCGAAACAAG | 432 to 413 (pVE46 tetA) | Amplification of tetA 5′ end and tetA promoter |
TETA2F | GTTGCTGGCGCCTATATCGC | 343 to 362 (pVE46 tetA) | Amplification of tetA 3′ end |
TETA2R | TCAGGTCGAGGTGGCCCGAC | 1176 to 1157 (pVE46 tetA) | Amplification of tetA 3′ end |
TETRF | TTCAGCTAGGTGACTTTGCT | −64 to −45 (pVE46 tetR) | Amplification of tetR |
TETRR | GTGCCTGACTGCGTTAGCAA | 746 to 727 (pVE46 tetR) | Amplification of tetR |
TETARTF | ACAATGCGCTCATCGTCATC | 11 to 30 (pVE46 tetA) | tetA RT-PCR |
TETARTR | GGCGCCTACAATCCATGC | 1125 to 1108 (pVE46 tetA) | tetA RT-PCR |
RP1TET1AF | CGGCTGCAACTTTGTCATGC | −123 to −104 (RP1 tetA) | Amplification of tetA 5′ end |
RP1TETA1R | CGCCGAAAATGACCCAAAGC | 712 to 693 (RP1 tetA) | Amplification of tetA 5′ end |
RP1TETA2F | CCGCTCAGCTTCGTTCGGTG | 595 to 614 (RP1 tetA) | Amplification of tetA 3′ end |
RP1TETA2R | CGATCAGCGATCGGCTCGTT | 1202 to 1183 (RP1 tetA) | Amplification of tetA 3′ end |
RP1TETRTETPROF | GCAGCGGTCCTGATCAATCG | 663 to 644 (RP1 tetR) | Amplification of tetA promoter and tetR |
RP1TETRTETPROR | CTCCGCTGGTCCGATTGAAC | −20 to −39 (RP1 tetA) | Amplification of tetA promoter and tetR |
RP1TETARTF | CGGCCAATCTTGCTCGTCTC | 217 to 236 (RP1 tetA) | tetA RT-PCR |
RP1TETARTR | CCTGCCTGGACAACATTGCT | 982 to 936 (RP1 tetA) | tetA RT-PCR |
Positions are relative to the start codon of the gene.