TABLE 2.
Predicted indices of helicity and experimental helix contentsa
Peptide | Index of helicity (AGADIR) | Helix contentb
|
|||
---|---|---|---|---|---|
Buffer
|
Liposomes
|
||||
− Salt | + Salt | − Salt | + Salt | ||
LLG | 0.97 | 13 | 11 | 12 | 10 |
GKE | 5.16 | 26 | 26 | 32 | 28 |
FKR | 2.93 | 26 | 27 | 37 | 38 |
Experiments performed in 10 mM Tris buffer (pH 7.4) in the presence (+ Salt) or absence (− Salt) of 150 mM NaCl.
Determined by circular dichroism spectroscopy of the peptides used in the study. Mean percent values of experiments performed in duplicate.