TABLE 1.
Primer sequences used for PCR mapping
| Amplicon (target) | Primer | Sequence (5′-3′) | Size of amplicon (bp) | Reference |
|---|---|---|---|---|
| A (orf513) | 341A | CGCCCACTCAAACAAACG | 468 | 15 |
| 341B | GAGGCTTTGGTGTAACCG | 15 | ||
| B (orf513-sul1) | 341STOP | ACATTAGTCGGCCAGCGG | 4,262 | 15 |
| SulR | GATTGCGCTTCGCAGATCTCCAGG | 12 | ||
| C (intI1-orf513) | intI1L | ACATGTGATGGCGACGCACGA | 3,943 | 12 |
| 341B | GAGGCTTTGGTGTAACCG | 15 | ||
| D (qnrB2-orf513) | qnrL | TGAACCACTGAACGTCGC | 2,696 | This study |
| 341B | GAGGCTTTGGTGTAACCG | 15 | ||
| E (sul1-dfrA19) | SulL | CCTGGAGATCTGCGAAGCGCAATC | 3,298 | This study |
| dfrR | CTCGCTGGCACTGGAAT | This study | ||
| F (orf513-aadA1) | 341STOP | ACATTAGTCGGCCAGCGG | 5,333 | 15 |
| aadAR | AAGTATGACGGGCTGATACTG | This study | ||
| G (dfrA19-aadA1) | dfrL | ATTCCAGTGCCAGCGAG | 3,531 | This study |
| aadAR | AAGTATGACGGGCTGATACTG | This study | ||
| H (oxa1-qacEΔ1) | oxaL | CTGAAATTGCTCAATTCAATAAAGC | 2,431 | This study |
| SulR2 | GTCGTTATAGCCCTATCTCGCGTC | This study |