TABLE 2.
Predicted genes on the 8,469-bp quinolone resistance plasmid pGNB2
| Gene | Position | Gene product | Function | Pfama | COGb | % Identityc | Reference |
|---|---|---|---|---|---|---|---|
| repC | 545-1426 | Putative replication protein C | Replication initiation | 06504 | 91 (RepC of plasmid p22K9 from Klebsiella pneumoniae) | 6 | |
| repA | 1413-2276 | Replicative helicase, NTPase | Replication initiation | 3598 | 84 (RepA of plasmid pRAS3.1 from Aeromonas salmonicida) | 18 | |
| orf1 | 2347-4788 | Relaxase, topoisomerase/primase | Mobilization | 03432, 01751 | 28 (BmgA from Bacteroides fragilis) | 2 | |
| mobC | 4772-5203 | Mobilization protein C | Mobilization | 05713 | 32 (MobC of plasmid pLJ42 from Lactobacillus plantarum) | AAZ13605 | |
| orf2 | 5661-6128 | Conserved hypothetical protein | Unknown | 35 (Lfe203p1 from Leptospirillum ferrooxidans) | 23 | ||
| orfI | 6680-7519 | Methyl-accepting chemotaxis protein | Putative function in signal transduction | 2202 | 95 (OrfI of Tn1721 on plasmid pFBAOT6 from Aeromonas punctata) | 27 | |
| qnrS2 | 7814-8469 | Pentapeptide repeat family protein | Quinolone resistance | 00805 | 1357 | 92 (QnrS of plasmid pAH0376 from Shigella flexneri) | 12 |
a
Pfam is a large collection of multiple sequence alignments and hidden Markov models covering many common protein domains and families.
b
The database of Clusters of Orthologous Groups of proteins (COG) phylogenetically classifies proteins.
c
The object of the percent identity comparison is given in parentheses.