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. 2003 May 5;100(10):6221–6226. doi: 10.1073/pnas.1031520100

Figure 4.

Figure 4

Aβ peptide accumulation is increased in insulysin-deficient mice. Aβ40 (A) and Aβ42 (B) were quantified by ELISA in brain diethylamine extracts. Two-way ANOVA revealed a significant effect of both IDE genotype and age on the steady-state levels of Aβ40 (P < 0.0001 for both effects) and Aβ42 (P < 0.001 and P = 0.002, respectively). The post hoc Student–Neuman–Keuls test indicated that, for both peptides, the mean values for the three different genotypes were statistically different from each other. In young, adult (8- to 10-wk) IDE+/+ (n = 3), IDE+/− (n = 3), and IDE−/− (n = 4) mice, the mean Aβ40 levels were 1.05, 1.40, and 1.70 pmol/g, respectively, and the mean Aβ42 levels were 0.39, 0.48, and 0.56 pmol/g. In the older (20-wk) IDE+/+ (n = 2), IDE+/− (n = 4), and IDE−/− (n = 3) mice, the mean Aβ40 levels were 1.36, 1.69, and 1.97 pmol/g, respectively, whereas the mean Aβ42 levels were 0.46, 0.53, and 0.59 pmol/g.