FIG. 1.

Recombinant generation of RNase 8 in E. coli. (A) Amino acid sequence of RNase 8 (single-letter code). Processing at the indicated putative cleavage site (arrow) leads to the mature RNase 8 sequence (boldface letters), which was recombinantly expressed in E. coli. The cysteyl residues presumably involved in disulfide bridges are underlined. (B) Strategy for expression of RNase 8 in E. coli. RNase 8 was expressed as a fusion protein containing an N-terminal His tag sequence. Cleavage of the expressed fusion protein with dipeptidyl aminopeptidase I results in the mature RNase 8 protein.