FIG. 2.

Hemolytic activity and salt sensitivity of RNase 8. (A) For analysis of hemolytic activity RNase 8 was incubated at 37°C for 3 h with 1 × 10⁹ human erythrocytes/ml in 10 mM sodium phosphate buffer (pH 7.4) containing 0.34 M sucrose. Hemolysis was determined by measuring the A₄₅₀ of the supernatants using 0.1% Triton X-100 for 100% hemolysis. (B) To test the salt sensitivity of RNase 8, E. coli (ATCC 35218) was treated with 3.5 μM RNase 8 in the presence of various concentrations of NaCl.