TABLE 1.
Antimicrobial activity of RNase 8 against various clinically relevant microorganisms
| Straina | MBCb (μM) | LD90 (μM) |
|---|---|---|
| Gram-positive strains | ||
| Staphylococcus aureus ATCC 6538 | 1.8 | 0.9 |
| Staphylococcus aureus ATCC 12600 | 1.8 | 0.4 |
| Staphylococcus aureus ATCC 33593 (MRSA) | 1.8 | 0.4 |
| Staphylococcus aureus ATCC 43300 (MRSA) | 0.9 | 0.2-0.4 |
| Staphylococcus epidermidis ATCC 14990 | 0.9-1.8 | 0.4-0.9 |
| Streptococcus pyogenes ATCC 12344 | 0.9-3.5 | 0.2-0.4 |
| Streptococcus pneumoniae ATCC 33400 | >7.0 | 3.5-7.0 |
| Enterococcus faecalis ATCC 29212 | 0.4-1.8 | 0.2 |
| Enterococcus faecalis ATCC 51299 (VRE) | 3.5 | 0.2-0.4 |
| Enterococcus faecium DSM 2146 | 0.4 | 0.2 |
| Enterococcus faecium clinical isolate (VRE) | 0.4 | 0.2 |
| Enterococcus faecium clinical isolate (VRE) | >7.0 | 0.2 |
| Enterococcus faecium clinical isolate | 0.4 | 0.2-0.4 |
| Gram-negative strains | ||
| Escherichia coli ATCC 25922 | 0.2-0.9 | 0.1-0.2 |
| Escherichia coli ATCC 35218 | 0.2 | 0.1 |
| Escherichia coli ATCC 11775 | 0.4 | 0.1-0.4 |
| Klebsiella pneumoniae ATCC 13882 | 0.2-0.9 | 0.1-0.4 |
| Klebsiella pneumoniae ATCC 13883 | 0.1-0.2 | 0.1 |
| Enterobacter cloacae ATCC 13047 | 7.0 | 0.4 |
| Serratia marcescens NCTC 10211 | >7.0 | >7.0 |
| Citrobacter freundii NCTC 9750 | >7.0 | 0.4-3.5 |
| Pseudomonas aeruginosa ATCC 10145 | 0.4-0.9 | 0.2-0.4 |
| Pseudomonas aeruginosa clinical isolate | 0.2 | 0.1 |
| Pseudomonas fluorescens NCTC 10038 | >7.0 | 0.9-1.8 |
| Burkholderia cepacia ATCC 25416 | >7.0 | >7.0 |
| Acinetobacter baumannii ATCC 19606 | 0.2 | 0.1 |
| Fungus, Candida albicans ATCC 24433 | 1.8 | 0.2 |
a
MRSA, methicillin-resistant Staphylococcus aureus; VRE, vancomycin-resistant enterococci.
b
MBC, minimal bactericidal concentration.