FIG. 1.

Direct detection of bacteriocin OR-7 from the crude antimicrobial preparation of Lactobacillus salivarius NRRL B-30514 culture after SDS-PAGE. Following SDS-PAGE, the gel was laid onto a plate seeded with Campylobacter jejuni to determine which band(s) corresponded to the antimicrobial activity and its specific molecular mass. Lane 1 contains molecular mass markers (in kilodaltons). The bands and zones of inhibition in lanes 2 and 4 are pure bacteriocin and a crude preparation demonstrating antimicrobial activity. Lane 3 is the crude antimicrobial preparation after adsorption with C. jejuni. The zone of growth inhibition (arrow) had a mass (M) of ∼6 kDa.