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Inhibition of yeast and fungal β-1,3-glucan synthase activity by purified scFvs. The membrane fractions were obtained from S. cerevisiae A451 (A) and C. albicans ATCC 10231 (B). The scFvs were added to the reaction mixture at a concentration of 25 μg/ml, as described in Materials and Methods. The activity was expressed in comparison to the activity of the sample without scFv and HM-1 as 100%. Data represent the mean of results for triplicate experiments ± standard error of the mean.
