TABLE 3.
IC50 values of recombinant scFv anti-idiotypic antibodies against the β-1,3-glucan synthase activity of four Candida speciesa
| scFv antibody | IC50 (10−8 M) for:
|
|||
|---|---|---|---|---|
| C. albicans ATCC 10231 | C. tropicalis NBRC 1400 | C. parapsilosis NBRC 1396 | C. glabrata NBRC 0622 | |
| ScFv-A1 | 19.7 | 16.5 | 11.7 | 4.2 |
| ScFv-A2 | 13.0 | 11.3 | 2.7 | 2.0 |
| ScFv-A3 | 12.7 | 20.7 | 22.7 | 11.4 |
| ScFv-A4 | 14.8 | 16.7 | 19.4 | 4.7 |
a
The measurement of IC50 values of β-1,3-glucan synthase activity of membrane fractions of Candida species is described in Materials and Methods.