TABLE 4.
Kinetic parameters for the binding of four scFv antibodies to nMAb-KT as measured by SPR analysisa
| Analyte | kon (105 M−1s−1) | koff (10−3 s−1) | KD (10−8 M) |
|---|---|---|---|
| scFv-A1 | 2.43 | 5.07 | 2.08 |
| scFv-A2 | 3.05 | 4.58 | 1.5 |
| scFv-A3 | 2.29 | 7.51 | 3.29 |
| scFv-A4 | 2.24 | 5.01 | 2.23 |
a
The interactions between HM-1 and the scFv antibodies with immobilized nMAb-KT were measured as described in Materials and Methods. kon, association rate constant; koff, dissociation rate constant; KD, dissociation constant. The constants were calculated by the equation KD = koff/kon.