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. 2006 Sep;50(9):2932–2940. doi: 10.1128/AAC.00134-06

FIG. 6.

FIG. 6.

Effects of PBP 10, LL37, magainin II, and mellitin peptides on RBCs. (A) Hemoglobin release from human red blood cells after the addition of mellitin (squares), magainin II (open diamonds), or calcium ionophore (triangles). (B and C) Hemoglobin release from RBCs after the addition of PBP 10 and LL37 peptides, respectively. In this experiment, RBCs were preincubated in the presence of EGTA (circles), calcium (diamonds), A23187 plus EGTA (triangles), or A23187 plus calcium (squares) for 20 min at 37°C before the peptides were added. (D) HI indicating osmotic fragility of red blood cells in the presence of LL37 (circles) and PBP 10 (triangles), measured as a change in the optical density at 625 nm in isotonic (open circles and open triangles) and hypotonic (filled circles and filled triangles) buffers. The data shown are means ± standard deviations of four or five experiments.