Figure 4.

Effects of the Gα isoform on channel deactivation. (A) Representative examples of current profiles obtained from HKIR3.1/3.2/A1 and HKIR3.1/3.2/M4 cell lines transiently transfected with G_iα₂-CFP and G_oα_A-CFP and voltage-clamped at −60 mV. The maximal concentration of agonist (1 μM NECA and 10 μM carbachol, respectively) was applied for 20 s (as indicated by the horizontal bar). (B) Summary of data obtained. Channel deactivation via both G_iα₂ and G_oα_A was significantly faster after stimulation of the M4 than the A₁ receptor.