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. 2003 Apr 28;100(10):6239–6244. doi: 10.1073/pnas.1037595100

Figure 5.

Figure 5

Differential effects of RGS8 on channel kinetics in response to receptor stimulation. (A) We recorded currents in HKIR3.1/3.2/M4 (Left), HKIR3.1/3.2/GGB (Center), and HKIR3.1/3.2/A1 (Right) cells voltage-clamped at −60 mV in response to 20-s agonist applications. (Upper) Control responses. (Lower) Responses in the presence of RGS8 (1 μg of cDNA) transiently transfected. (B) Summary of kinetic data in the absence (Control) and presence (+RGS8) of RGS8. In the HKIR3.1/3.2/M4 cell line, RGS8 affects only the deactivation kinetics but not the activation kinetics, whereas in the HKIR3.1/3.2/GGB cell line it significantly accelerates both activation and deactivation parameters; in the HKIR3.1/3.2/A1 cell line RGS8 has no effects at all. NS, not significant; ttp, time to peak.