TABLE 2.
Drug susceptibilities and organic solvent tolerances of E. coli strains with or without marA or marA47Yp
| Straina | MIC of drug (μg/ml)b
|
Growth on solventc
|
||||||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| Tet
|
Dox
|
Rif
|
Chl
|
Nor
|
Nal
|
Hexane
|
Cyclohexane
|
Pentane
|
||||||||||
| − | + | − | + | − | + | − | + | − | + | − | + | − | + | − | + | − | + | |
| AG100 | 1.0 | 1.0 | 1.5 | 1.5 | 7.5 | 7.5 | 4.0 | 4.0 | .08 | .08 | 3.5 | 3.5 | +++ | +++ | − | − | − | − |
| AG112 | 6.0 | 6.0 | 6.0 | 6.0 | 9.0 | 9.0 | 10 | 10 | .25 | 0.25 | 6.0 | 6.0 | +++ | +++ | +++ | +++ | ++ | ++ |
| AG100/v | 1.0 | 1.0 | 1.5 | 1.5 | 7.5 | 7.5 | 4.0 | 4.0 | .08 | .08 | 3.5 | 3.5 | +++ | +++ | − | − | − | − |
| AG100/102 | 1.0 | 2.0 | 1.5 | 2.5 | 7.5 | 8.0 | 4.0 | 6.0 | .08 | 0.15 | 3.5 | 4.5 | +++ | +++ | − | +++ | − | − |
| AG100/RU1 | 1.0 | 2.0 | 1.5 | 2.5 | 7.5 | 8.0 | 4.0 | 6.0 | .08 | 0.15 | 3.5 | 4.5 | +++ | +++ | − | +++ | − | + |
| AG100Kan | 1.0 | 1.0 | 1.5 | 1.5 | 7.5 | 7.5 | 4.0 | 4.0 | .08 | .08 | 3.0 | 3.0 | +++ | +++ | − | − | − | − |
| AG100Kan/v | 1.0 | 1.0 | 1.5 | 1.5 | 7.5 | 7.5 | 4.0 | 4.0 | .08 | .08 | 3.0 | 3.0 | +++ | +++ | − | − | − | − |
| AG100Kan/102 | 1.0 | 2.0 | 1.5 | 2.5 | 7.5 | 8.0 | 4.0 | 5.0 | .08 | 0.15 | 3.0 | 4.0 | +++ | +++ | − | ++ | − | − |
| AG100Kan/RU1 | 1.0 | 2.0 | 1.5 | 2.5 | 7.5 | 8.0 | 4.0 | 5.0 | .08 | 0.15 | 3.0 | 4.0 | +++ | +++ | − | ++ | − | + |
| AG100A | 0.3 | 0.3 | 0.3 | 0.3 | 6.0 | 6.0 | 1.5 | 1.5 | ND | ND | ND | ND | − | − | − | − | − | − |
| AG100A/v | 0.3 | 0.3 | 0.3 | 0.3 | 6.0 | 6.0 | 1.5 | 1.5 | ND | ND | ND | ND | − | − | − | − | − | − |
| AG100A/102 | 0.3 | 0.3 | 0.3 | 0.3 | 6.0 | 6.0 | 1.5 | 1.5 | ND | ND | ND | ND | − | − | − | − | − | − |
| AG100A/RU1 | 0.3 | 0.3 | 0.3 | 0.3 | 6.0 | 6.0 | 1.5 | 1.5 | ND | ND | ND | ND | − | − | − | − | − | − |
| N8453 | 0.5 | 0.5 | 0.6 | 0.6 | 7.0 | 7.0 | ND | ND | .01 | 0.04 | 3.0 | 3.0 | − | − | − | − | − | − |
| N8453/v | 0.5 | 0.5 | 0.6 | 0.6 | 7.0 | 7.0 | ND | ND | .01 | 0.04 | 3.0 | 3.0 | − | − | − | − | − | − |
| N8453/102 | 0.5 | 0.8 | 0.6 | 1.5 | 7.0 | 8.0 | ND | ND | .01 | 0.05 | 3.0 | 4.0 | − | +++ | − | + | − | − |
| N8453/RU1 | 0.5 | 1.0 | 0.6 | 1.6 | 7.0 | 8.0 | ND | ND | .01 | 0.05 | 3.0 | 4.0 | − | +++ | − | ++ | − | + |
AG100, wild type; AG112, marR mutant; AG100Kan, Δmar locus; AG100A, ΔacrAB; N8453, Δmar, sox rob; 102, plasmid pMB102; RU1, plasmid pRU1; v, pJPBH (vector control). See Table 1 for strain descriptions. N8453 has a chloramphenicol resistance transposon.
Minus or plus sign, absence or presence of 0.2 mM IPTG; Tet, tetracycline; Dox, doxycycline; Rif, rifampicin; Chl, chloramphenicol; Nor, norfloxacin; Nal, nalidixic acid; ND, not determined. Reproducibly observed increased MICs (compared to those for parental strains) are in boldface. Results are averages for experiments performed in triplicate.
Growth compared to growth on medium without organic solvents: −, no growth; +, minimal growth; ++, moderate growth; +++, full growth.