FIG. 2.

Surface antigen expression on human monocytes treated with different doses of S. gordonii. Monocytes, isolated from buffy coats of healthy donors using magnetic CD14-conjugated beads, were cultured with S. gordonii at a concentration of 1 or 10 bacteria per cell (▪) or in medium alone (□). Cells were harvested after 18 h and analyzed by flow cytometry. The mean fluorescence intensity minus the fluorescence of the matched-isotype control antibody (ΔMFI) was calculated for each sample. Values are reported as means ± standard deviations from three independent experiments. *, P ≤ 0.05; **, P ≤ 0.01, compared to results for untreated cells.