TABLE 2.
Comparison of bovine DC surface phenotypes
| Surface molecule | Surface phenotypea
|
|||
|---|---|---|---|---|
| Spleen DCsb | Peripheral blood DCsb | Afferent lymph DC subset 1c | Afferent lymph DC subset 2c | |
| MHC class II | ++ | ++ | ++ | ++ |
| CD1b | − | − | + | ++ |
| CD2 | − | − | − | − |
| CD4 | − | − | − | − |
| CD8 | − | − | − | − |
| CD11a | ++ | ++ | + | − |
| CD11b | ± | + | − | − |
| CD11c | ++ | + | ± | + |
| CD13 | ++ | − | + | − |
| CD21 | − | − | − | − or + |
| CD45RO | − | − | + | + |
| CD80 | ± | ± | ++ | ++ |
| CD86 | − | − | ++ | ++ |
| CD172a | + | + | − | + |
| CD205 | ++ | + | ++ | ++ |
a
Intensities of monoclonal antibody binding are indicated as − (negative), ± (weak), + (positive), or ++ (strongly positive), according to the conventions of Howard and Hope (13). In this study, these symbols are defined as follows: ++, ≥60% of cells were positive; +, 15 to 59% of cells were positive; ±, <15% were positive; and −, cells were negative.
b
Splenic and peripheral blood DCs were analyzed as uncultured cells using three-color flow cytometry.